Students and a professor performing research with lasers.

2D TOCSY

  1. Dipsi2esgpph data

    Go to an existing 2D-Tocsy experiment (pulse program: dipsi2esfbgpph), type “edc” to copy its parameters and generate a new experiment number. Or in a new experiment, "rpar DIPSI2ESFBGPPH".

    Pulse program dipsi2esfbgpph is based on dipsi2esgpph and have f2 and f3 channels included.

  2. Type "edasp" to check /adjust spectrometer routing.

  3. Type “eda”, set parameters td, ns, ds, sw, O1P, O2P and O3P if necessary.

  4. In acquisition window, if sample is unlabeled, leave ZGOPTNS blank. If sample is N15 labeled, set ZGOPTNS "-DLABEL_N". If sample is N15 and C13 labeled, set ZGOPTNS "-DLABEL_CN".

  5. Type “ased”, adjust power length and power level for 1H. If sample is labeled, you will need to check 15N and/or 13C power level and power length according. Or type "getprosol 1H A B" to set power related parameters. A is the pulse length in µs, and B is the power leverl in dB.

  6. Type "pulse" to calculate pL10 based on targeting p6 value. Use p6 35 us.

  7. Set d9 (Tocsy mixing time) 90 ms for a protein sample; set pL32 70dB for water presaturation

  8. In gs mode, adjust O1P and p12 to achieve minimum FID area integral for water suppression.

  9. Start the experiment by rga and zg.

  10. Process the FID by typing “xfb”. Phase the spectrum when necessary.

2DTOCSY, NC-Ub, 0.9mM, d9=90ms, expt 2h 33 min