1D 31P

1D 31P

  1. Type "rpar P31CPD" in a new data set, or use "edc" command to copy from an exisiting 1D 31P experiment. Another option is "rpar P31" depending on decoupling choice.

  2. Use "getprosol" command to load the default phosphorous power parameters.

  3. In the acquisition parameter window check and adjust td, nsds, sw, d1.

  4. Estimate receiver gain using command rga, then start the experiment using zg command;

  5. Process the FID by typing “efp”, and phase the spectrum when necessary.

1D P31 Spectrum of a 6-mer DNA